antioxidant and protective effects of angipars™, a new herbal drug, on hydrogen peroxide-induced oxidative stress in hepg2 and a549 cell lines

in the present study, free-radical-scavenging and antioxidant activities of angipars™, an herbal extract which has been registered as a therapeutic drug for diabetic foot ulcer, were examined. we investigated the protective effect of angipars™ on cytotoxicity produced by exposure to h2o2 (300 µm) in hepg2 and a549 cell lines, using mtt assay, glutathione (gsh) content analysis, and lipid peroxidation evaluation. angipars™ concentrations of 1 - 20 µg/ml effectively scavenged dpph radicals in a dose-dependent manner, while hydroxyl-radical-scavenging activity was only observed at high doses. following a 4-hour cell treatment with h2o2, a noticeable decline in cell viability was observed, which was alleviated by angipars™ (pre-incubated for 24 hours) at high doses. angipars™ significantly reduced gsh content and products of lipid peroxidation, resulting from h2o2 exposure. these findings suggest that angipars™ exhibits positive antioxidant activity in vitro and can attenuate h2o2-induced cytotoxicity; in fact, this may represent the cellular mechanisms for its cytoprotective activity.